【mIL 10】重組小鼠白介素-10
產(chǎn)品報價
¥800/2μg ¥2080/10μg ¥74880/1mg
背景資料
IL10 is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.
產(chǎn)品描述
小鼠白介素-10重組蛋白是在大腸桿菌中重組表達的,是一條單鏈、非糖基化的多肽鏈,包含有161個氨基酸,分子量為18785Da。該小鼠白介素-10重組蛋白最終由標準的色譜技術(shù)純化出。
來源宿主
大腸桿菌中重組表達
序列
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Arg-Gly-Gln.
溶解建議
提供的小鼠白介素-10(mIL 10)產(chǎn)品為過濾滅菌的白色凍干粉末;該凍干粉末是從含小鼠白介素-10(mIL 10)的PBS溶液中經(jīng)進一步透析并凍干產(chǎn)生的。我們建議加無菌的18MΩ-cm水以配制工作儲存液到100μg/ml,后繼實驗過程中可根據(jù)實驗要求進一步稀釋。
保存建議
小鼠白介素-10(mIL 10)在室溫可保存3周,或置于-18℃凍存用于長期保存。重新溶解后可以在4℃冷藏保存至少2-7天;如果想延長保存時間,建議分裝(加載體蛋白如0.1% HSA或 BSA)后置于-18℃凍存。請避免反復(fù)凍融。
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